total p44 Search Results


67404 illkirch  (Cell Signaling Technology Inc)
88
Cell Signaling Technology Inc 67404 illkirch
67404 Illkirch, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/67404 illkirch/product/Cell Signaling Technology Inc
Average 88 stars, based on 1 article reviews
67404 illkirch - by Bioz Stars, 2026-03
88/100 stars
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total p44 42 mapk erk1 2 sandwich elisa kit  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc total p44 42 mapk erk1 2 sandwich elisa kit
Effect of AKG on phosphorylation of MAP kinases and the influence of the selective JNK inhibitor (SP600125) on AKG-induced apoptosis in Saos-2 cells. The cells were incubated without or with AKG for 6 h and 24 h, and phosphorylated and total <t>ERK1/2,</t> JNK and p38 levels were determined with the ELISA assay. Quantification of the amounts of phosphorylated to total MAP kinases ( A – C ). The cells were treated with 50 mM AKG without or with SP600125 (5 μM) and harvested after 72 h of treatment for apoptosis analysis. The representative dot plots indicate the percentage of An − /PI + necrotic cells (Q1), An + /PI + late apoptotic cells (Q2), An − /PI − viable cells (Q3), and An + /PI − early apoptotic cells (Q4) in the AKG or/and SP600125-treated Saos-2 cell cultures ( D ). Quantification of the amounts of phosphorylated to total JKN kinase ( E ) and histogram representation of the quantitative percentage of apoptotic (early + late apoptosis) cells ( F ) in the control, SP600125, AKG, and SP600125 + AKG-treated Saos-2 cell cultures. Data are expressed as means ± SD for three independent experiments. ** p < 0.01, *** p < 0.001 in comparison to the control; one-way ANOVA test.
Total P44 42 Mapk Erk1 2 Sandwich Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total p44 42 mapk erk1 2 sandwich elisa kit/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
total p44 42 mapk erk1 2 sandwich elisa kit - by Bioz Stars, 2026-03
93/100 stars
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total p44/42 map kinase (ab-204  (Signalway Antibody)
90
Signalway Antibody total p44/42 map kinase (ab-204
Effect of AKG on phosphorylation of MAP kinases and the influence of the selective JNK inhibitor (SP600125) on AKG-induced apoptosis in Saos-2 cells. The cells were incubated without or with AKG for 6 h and 24 h, and phosphorylated and total <t>ERK1/2,</t> JNK and p38 levels were determined with the ELISA assay. Quantification of the amounts of phosphorylated to total MAP kinases ( A – C ). The cells were treated with 50 mM AKG without or with SP600125 (5 μM) and harvested after 72 h of treatment for apoptosis analysis. The representative dot plots indicate the percentage of An − /PI + necrotic cells (Q1), An + /PI + late apoptotic cells (Q2), An − /PI − viable cells (Q3), and An + /PI − early apoptotic cells (Q4) in the AKG or/and SP600125-treated Saos-2 cell cultures ( D ). Quantification of the amounts of phosphorylated to total JKN kinase ( E ) and histogram representation of the quantitative percentage of apoptotic (early + late apoptosis) cells ( F ) in the control, SP600125, AKG, and SP600125 + AKG-treated Saos-2 cell cultures. Data are expressed as means ± SD for three independent experiments. ** p < 0.01, *** p < 0.001 in comparison to the control; one-way ANOVA test.
Total P44/42 Map Kinase (Ab 204, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total p44/42 map kinase (ab-204/product/Signalway Antibody
Average 90 stars, based on 1 article reviews
total p44/42 map kinase (ab-204 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Effect of AKG on phosphorylation of MAP kinases and the influence of the selective JNK inhibitor (SP600125) on AKG-induced apoptosis in Saos-2 cells. The cells were incubated without or with AKG for 6 h and 24 h, and phosphorylated and total ERK1/2, JNK and p38 levels were determined with the ELISA assay. Quantification of the amounts of phosphorylated to total MAP kinases ( A – C ). The cells were treated with 50 mM AKG without or with SP600125 (5 μM) and harvested after 72 h of treatment for apoptosis analysis. The representative dot plots indicate the percentage of An − /PI + necrotic cells (Q1), An + /PI + late apoptotic cells (Q2), An − /PI − viable cells (Q3), and An + /PI − early apoptotic cells (Q4) in the AKG or/and SP600125-treated Saos-2 cell cultures ( D ). Quantification of the amounts of phosphorylated to total JKN kinase ( E ) and histogram representation of the quantitative percentage of apoptotic (early + late apoptosis) cells ( F ) in the control, SP600125, AKG, and SP600125 + AKG-treated Saos-2 cell cultures. Data are expressed as means ± SD for three independent experiments. ** p < 0.01, *** p < 0.001 in comparison to the control; one-way ANOVA test.

Journal: International Journal of Molecular Sciences

Article Title: Alpha Ketoglutarate Exerts In Vitro Anti-Osteosarcoma Effects through Inhibition of Cell Proliferation, Induction of Apoptosis via the JNK and Caspase 9-Dependent Mechanism, and Suppression of TGF-β and VEGF Production and Metastatic Potential of Cells

doi: 10.3390/ijms21249406

Figure Lengend Snippet: Effect of AKG on phosphorylation of MAP kinases and the influence of the selective JNK inhibitor (SP600125) on AKG-induced apoptosis in Saos-2 cells. The cells were incubated without or with AKG for 6 h and 24 h, and phosphorylated and total ERK1/2, JNK and p38 levels were determined with the ELISA assay. Quantification of the amounts of phosphorylated to total MAP kinases ( A – C ). The cells were treated with 50 mM AKG without or with SP600125 (5 μM) and harvested after 72 h of treatment for apoptosis analysis. The representative dot plots indicate the percentage of An − /PI + necrotic cells (Q1), An + /PI + late apoptotic cells (Q2), An − /PI − viable cells (Q3), and An + /PI − early apoptotic cells (Q4) in the AKG or/and SP600125-treated Saos-2 cell cultures ( D ). Quantification of the amounts of phosphorylated to total JKN kinase ( E ) and histogram representation of the quantitative percentage of apoptotic (early + late apoptosis) cells ( F ) in the control, SP600125, AKG, and SP600125 + AKG-treated Saos-2 cell cultures. Data are expressed as means ± SD for three independent experiments. ** p < 0.01, *** p < 0.001 in comparison to the control; one-way ANOVA test.

Article Snippet: The quantification of the intracellular levels of total and phosphorylated JNK, ERK1/2, p38, and AKT kinases and the contents of cyclin D1 and p21 proteins in the treated cells was carried out using the PathScan ® ELISA kits: Total SAPK/JNK Sandwich ELISA Kit, Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Kit, Total p44/42 MAPK (Erk1/2) Sandwich ELISA Kit, Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit, Phospho-p38 MAPK (Thr180/Tyr182) Sandwich ELISA Kit, Total Cyclin D1 Sandwich ELISA Kit, Total p21 Waf1/Cip1 Sandwich ELISA Kit (Cell Signaling Technology Danvers, MA, USA), and p38 MAPK alpha ELISA Kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions as described previously [ ].

Techniques: Incubation, Enzyme-linked Immunosorbent Assay